The first aim of my final project is to develop a rapid, multiplex genetic biosensor that detects pathological α-synuclein and Parkinson’s disease-associated microRNAs by utilizing synthetic gene circuits, colorimetric reporters, and lateral flow assay formats.

I will use:

• OpenTrons protocols for DNA assembly and transformation
• Synthetic biology tools like Benchling, SnapGene, and NUPACK for gene circuit design
• Colorimetric reporters such as lacZ/X-gal, chromogenic enzymes, or G-quadruplex DNAzymes
• Twist Bioscience for ordering synthetic gene constructs
• Lateral flow device (LFD) assembly guides and protocols for integrating components into a paper-based tes

The second aim of my project is to validate and optimize the multiplex diagnostic system in biological samples (blood) and ensure high specificity and sensitivity for early-stage Parkinson’s disease.

• Testing the sensor in spiked biofluids to evaluate real-world performance

• Comparing results with known clinical biomarkers and controls

• Improving component modularity for the potential detection of other neurodegenerative disease markers

  This step builds on the first aim by applying the proof-of-concept system in near-clinical conditions and refining its robustness and ease of use.

The third aim of my project is to create a low-cost, portable, and multiplex diagnostic platform that transforms the early detection and stratification of neurodegenerative diseases, especially in underserved or resource-limited settings.

• Early intervention and monitoring before irreversible neurodegeneration

• Widespread, accessible screening for Parkinson’s and related disorders

• A shift in clinical practice by enabling molecular diagnosis at the point of care, moving beyond symptom-based diagnosis

  Ultimately, this diagnostic tool could contribute to global health equity in neurodegenerative disease care and prevention.